Addgene inc pontus aspenström
Pontus Aspenström, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prk5+myc+miro2/pmc05067282__mmc2-173-18-20?v=Addgene+inc
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pontus aspenström - by Bioz Stars, 2026-08

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mycmiro2∆tm (Addgene inc)

Addgene inc mycmiro2∆tm
Mycmiro2∆Tm, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mycmiro2∆tm - by Bioz Stars, 2026-08

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prk5 myc miro2 (Addgene inc)

Addgene inc prk5 myc miro2
$MYO19898–970 localizes to mitochondria in the presence of <t>Miro2</t> and has more rapid exchange kinetics than constructs containing the putative membrane-insertion motif. (a) Mito/cyto ratio was used to quantify the enhancement of mitochondrial localization of MYO19-GFP constructs in the presence of mchr-Miro2. While coexpression of mchr-Miro2 with MYO19898–970-GFP enhanced the GFP-localization on mitochondria, mitochondrial localization of MYO19853–935-GFP and MYO19860–890-GFP were not enhanced by the coexpression of mchr-Miro2 (*p<0.001, t-test between MYO19898–970-GFP and MYO19898–970-GFP coexpressed with mchr-Miro2). (b) Coexpression of mchr-Miro2 decreased the fraction of the population of MYO19898–970-GFP expressing cells displaying a cytosolic staining pattern for GFP, but expression of mchr-Miro2 did not alter the population of MYO19853–935-GFP or MYO19860–890-GFP expressing cells with a cytosolic GFP pattern, further implicating Miro2 in localizing MYO19 to mitochondria (*p < 0.05, compared to MYO19898–970-GFP, Tukey analysis). (c) FRAP recovery of MYO19853–935-GFP and MYO19860–890-GFP expressing cells is relatively slow with a large immobile fraction, when compared to MYO19898–970-GFP in cells expressing mchr-Miro2, indicating that the mechanism of interaction with mitochondria for these two regions of MYO19 differs. (d) Same data as in (c), but plotted on a different time scale to show the complete time course for the longer recovery kinetics. FRAP kinetic parameters are listed in Table 1$
Prk5 Myc Miro2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prk5+myc+miro2/pmc08556674-446-52-53?v=Addgene+inc
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prk5 myc miro2 - by Bioz Stars, 2026-08

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prk5 myc miro2 e208k e328k (Addgene inc)

Addgene inc prk5 myc miro2 e208k e328k
$MYO19898–970 localizes to mitochondria in the presence of <t>Miro2</t> and has more rapid exchange kinetics than constructs containing the putative membrane-insertion motif. (a) Mito/cyto ratio was used to quantify the enhancement of mitochondrial localization of MYO19-GFP constructs in the presence of mchr-Miro2. While coexpression of mchr-Miro2 with MYO19898–970-GFP enhanced the GFP-localization on mitochondria, mitochondrial localization of MYO19853–935-GFP and MYO19860–890-GFP were not enhanced by the coexpression of mchr-Miro2 (*p<0.001, t-test between MYO19898–970-GFP and MYO19898–970-GFP coexpressed with mchr-Miro2). (b) Coexpression of mchr-Miro2 decreased the fraction of the population of MYO19898–970-GFP expressing cells displaying a cytosolic staining pattern for GFP, but expression of mchr-Miro2 did not alter the population of MYO19853–935-GFP or MYO19860–890-GFP expressing cells with a cytosolic GFP pattern, further implicating Miro2 in localizing MYO19 to mitochondria (*p < 0.05, compared to MYO19898–970-GFP, Tukey analysis). (c) FRAP recovery of MYO19853–935-GFP and MYO19860–890-GFP expressing cells is relatively slow with a large immobile fraction, when compared to MYO19898–970-GFP in cells expressing mchr-Miro2, indicating that the mechanism of interaction with mitochondria for these two regions of MYO19 differs. (d) Same data as in (c), but plotted on a different time scale to show the complete time course for the longer recovery kinetics. FRAP kinetic parameters are listed in Table 1$
Prk5 Myc Miro2 E208k E328k, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prk5+myc+miro2/pmc04642244-374-17-22?v=Addgene+inc
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prk5-myc-miro2 r425v (plasmid #47898) (Addgene inc)

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Standard format: Plasmid sent in bacteria as agar stab

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prk5-myc-miro2 s430n (plasmid #47899) (Addgene inc)

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Standard format: Plasmid sent in bacteria as agar stab

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prk5-myc-miro2 a13v (plasmid #47896) (Addgene inc)

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Standard format: Plasmid sent in bacteria as agar stab

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Image Search Results

$MYO19898–970 localizes to mitochondria in the presence of Miro2 and has more rapid exchange kinetics than constructs containing the putative membrane-insertion motif. (a) Mito/cyto ratio was used to quantify the enhancement of mitochondrial localization of MYO19-GFP constructs in the presence of mchr-Miro2. While coexpression of mchr-Miro2 with MYO19898–970-GFP enhanced the GFP-localization on mitochondria, mitochondrial localization of MYO19853–935-GFP and MYO19860–890-GFP were not enhanced by the coexpression of mchr-Miro2 (*p<0.001, t-test between MYO19898–970-GFP and MYO19898–970-GFP coexpressed with mchr-Miro2). (b) Coexpression of mchr-Miro2 decreased the fraction of the population of MYO19898–970-GFP expressing cells displaying a cytosolic staining pattern for GFP, but expression of mchr-Miro2 did not alter the population of MYO19853–935-GFP or MYO19860–890-GFP expressing cells with a cytosolic GFP pattern, further implicating Miro2 in localizing MYO19 to mitochondria (*p < 0.05, compared to MYO19898–970-GFP, Tukey analysis). (c) FRAP recovery of MYO19853–935-GFP and MYO19860–890-GFP expressing cells is relatively slow with a large immobile fraction, when compared to MYO19898–970-GFP in cells expressing mchr-Miro2, indicating that the mechanism of interaction with mitochondria for these two regions of MYO19 differs. (d) Same data as in (c), but plotted on a different time scale to show the complete time course for the longer recovery kinetics. FRAP kinetic parameters are listed in Table 1$

Journal: Cytoskeleton (Hoboken, N.J.)

Article Title: The MyMOMA domain of MYO19 encodes for distinct Miro-dependent and Miro-independent mechanisms of interaction with mitochondrial membranes

doi: 10.1002/cm.21560

Figure Lengend Snippet: MYO19898–970 localizes to mitochondria in the presence of Miro2 and has more rapid exchange kinetics than constructs containing the putative membrane-insertion motif. (a) Mito/cyto ratio was used to quantify the enhancement of mitochondrial localization of MYO19-GFP constructs in the presence of mchr-Miro2. While coexpression of mchr-Miro2 with MYO19898–970-GFP enhanced the GFP-localization on mitochondria, mitochondrial localization of MYO19853–935-GFP and MYO19860–890-GFP were not enhanced by the coexpression of mchr-Miro2 (*p<0.001, t-test between MYO19898–970-GFP and MYO19898–970-GFP coexpressed with mchr-Miro2). (b) Coexpression of mchr-Miro2 decreased the fraction of the population of MYO19898–970-GFP expressing cells displaying a cytosolic staining pattern for GFP, but expression of mchr-Miro2 did not alter the population of MYO19853–935-GFP or MYO19860–890-GFP expressing cells with a cytosolic GFP pattern, further implicating Miro2 in localizing MYO19 to mitochondria (*p < 0.05, compared to MYO19898–970-GFP, Tukey analysis). (c) FRAP recovery of MYO19853–935-GFP and MYO19860–890-GFP expressing cells is relatively slow with a large immobile fraction, when compared to MYO19898–970-GFP in cells expressing mchr-Miro2, indicating that the mechanism of interaction with mitochondria for these two regions of MYO19 differs. (d) Same data as in (c), but plotted on a different time scale to show the complete time course for the longer recovery kinetics. FRAP kinetic parameters are listed in Table 1

Article Snippet: GFP-Cytob 5 RR was a gift from Nica Borgese ( Borgese, Gazzoni, Barberi, Colombo, & Pedrazzini, 2001 ). myc-BioID2-MCS was a gift from Kyle Roux ( Kim et al., 2016 ) (Addgene plasmid # 74223; http://n2t.net/addgene:74223 ; RRID:Addgene_74223). mCherry-LaminA-C-18 was a gift from Michael Davidson (Addgene plasmid # 55068; http://n2t.net/addgene:55068 ; RRID:Addgene_55068). pRK5-myc-Miro2 (Addgene plasmid # 47891; http://n2t.net/addgene:47891 ; RRID:Addgene_47891), pRK5-myc-Miro2 T18N (Addgene plasmid # 47897; http://n2t.net/addgene:47897 ; RRID:Addgene_47897), and pRK5-myc-Miro2 Δ593–618 (Addgene plasmid # 47901; http://n2t.net/addgene:47901 ; RRID:Addgene_47901) were gifts from Pontus Aspenström ( Fransson et al., 2003 , 2006 ).

Techniques: Construct, Expressing, Staining

FRAP kinetic analysis for proteins used in this study

Journal: Cytoskeleton (Hoboken, N.J.)

Article Title: The MyMOMA domain of MYO19 encodes for distinct Miro-dependent and Miro-independent mechanisms of interaction with mitochondrial membranes

doi: 10.1002/cm.21560

Figure Lengend Snippet: FRAP kinetic analysis for proteins used in this study

Techniques:

PARF kinetic analysis for proteins used in this study

Journal: Cytoskeleton (Hoboken, N.J.)

Article Title: The MyMOMA domain of MYO19 encodes for distinct Miro-dependent and Miro-independent mechanisms of interaction with mitochondrial membranes

doi: 10.1002/cm.21560

Figure Lengend Snippet: PARF kinetic analysis for proteins used in this study

Techniques:

$A subset of well-conserved, charged residues are essential for Miro2-dependent localization of MYO19898–970. (a) Clustal Omega multiple sequence alignments of the MYO19 MyMOMA domain and the Miro2 N-terminal GTPase domain identified well-conserved, charged residues in each domain, which were mutated to alanine to determine their contribution to the MYO19/Miro2 interaction. (b) Mutating either the basic residues in MYO19898–970-GFP to alanine or the acidic residues in mchr-Miro2 to alanine decreased the localization of MYO19898–970-GFP to mitochondria, as determined by the mito/cyto ratio (*p < 0.05, Dunnett’s test vs. MYO19898–970-GFP wild type coexpressed with mchr-Miro2 wild type). (c) Mutations similarly decreased the fraction of cells displaying a cytosolic staining pattern for GFP, whether those mutations were to MYO19 or Miro2 (*p < 0.05, Dunnett’s test vs. MYO19898–970-GFP wild type coexpressed with mchr-Miro2 wild type). (d) FRAP analysis of MYO19898–970-GFP mutant shows faster exchange kinetics than wild type, consistent with basic residues in the MYO19 mediating interactions with Miro2. The exchange kinetics for wild type MYO19898–970-GFP with the mutant mchr-Miro2 construct were similar to exchange kinetics with the wild type mchr-Miro2 wild type construct. This is likely due to endogenous Miro recruiting MYO19898–970-GFP. Multiple sequence alignments can be found in the Figure S4. Numbers at the base of the bars indicate the number of replicates. Error bars represent SEM. FRAP kinetic parameters are listed in Table 1$

Journal: Cytoskeleton (Hoboken, N.J.)

Article Title: The MyMOMA domain of MYO19 encodes for distinct Miro-dependent and Miro-independent mechanisms of interaction with mitochondrial membranes

doi: 10.1002/cm.21560

Figure Lengend Snippet: A subset of well-conserved, charged residues are essential for Miro2-dependent localization of MYO19898–970. (a) Clustal Omega multiple sequence alignments of the MYO19 MyMOMA domain and the Miro2 N-terminal GTPase domain identified well-conserved, charged residues in each domain, which were mutated to alanine to determine their contribution to the MYO19/Miro2 interaction. (b) Mutating either the basic residues in MYO19898–970-GFP to alanine or the acidic residues in mchr-Miro2 to alanine decreased the localization of MYO19898–970-GFP to mitochondria, as determined by the mito/cyto ratio (*p < 0.05, Dunnett’s test vs. MYO19898–970-GFP wild type coexpressed with mchr-Miro2 wild type). (c) Mutations similarly decreased the fraction of cells displaying a cytosolic staining pattern for GFP, whether those mutations were to MYO19 or Miro2 (*p < 0.05, Dunnett’s test vs. MYO19898–970-GFP wild type coexpressed with mchr-Miro2 wild type). (d) FRAP analysis of MYO19898–970-GFP mutant shows faster exchange kinetics than wild type, consistent with basic residues in the MYO19 mediating interactions with Miro2. The exchange kinetics for wild type MYO19898–970-GFP with the mutant mchr-Miro2 construct were similar to exchange kinetics with the wild type mchr-Miro2 wild type construct. This is likely due to endogenous Miro recruiting MYO19898–970-GFP. Multiple sequence alignments can be found in the Figure S4. Numbers at the base of the bars indicate the number of replicates. Error bars represent SEM. FRAP kinetic parameters are listed in Table 1

Techniques: Sequencing, Staining, Mutagenesis, Construct

$Miro2-dependent localization of MYO19898–970 may depend on the nucleotide state of Miro2. (a) Point mutations made to the N-terminal domain of Miro2 are thought to result in the GTPase being in the GTP-bound (A13V) or GDP-bound (T18N) state. (b) Expressing the putative GDP-bound mchr-Miro2 T18N mutant decreased the localization of MYO19898–970-GFP to mitochondria while the putative GTP-bound A13V mutant did not, as determined by the mito/cyto ratio (*p < 0.05, Dunnett’s test vs. MYO19898–970-GFP coexpressed with mchr-Miro2 wild type). (c) Expression of mchr-Miro2 T18N increased the fraction of cells displaying a cytosolic staining pattern for GFP (*p < 0.05, Dunnett’s test vs. MYO19898–970-GFP coexpressed with mchr-Miro2 wild type). (d) FRAP analysis of MYO19898–970-GFP with wild type or GTP-state mutants shows similar exchange kinetics of MYO19898–970-GFP for all three conditions. For the mchr-Miro2 T18N, this is likely due to endogenous Miro recruiting MYO19898–970-GFP. Numbers at the base of the bars indicate the number of replicates. Error bars represent SEM. FRAP kinetic parameters are listed in Table 1$

Journal: Cytoskeleton (Hoboken, N.J.)

Article Title: The MyMOMA domain of MYO19 encodes for distinct Miro-dependent and Miro-independent mechanisms of interaction with mitochondrial membranes

doi: 10.1002/cm.21560

Figure Lengend Snippet: Miro2-dependent localization of MYO19898–970 may depend on the nucleotide state of Miro2. (a) Point mutations made to the N-terminal domain of Miro2 are thought to result in the GTPase being in the GTP-bound (A13V) or GDP-bound (T18N) state. (b) Expressing the putative GDP-bound mchr-Miro2 T18N mutant decreased the localization of MYO19898–970-GFP to mitochondria while the putative GTP-bound A13V mutant did not, as determined by the mito/cyto ratio (*p < 0.05, Dunnett’s test vs. MYO19898–970-GFP coexpressed with mchr-Miro2 wild type). (c) Expression of mchr-Miro2 T18N increased the fraction of cells displaying a cytosolic staining pattern for GFP (*p < 0.05, Dunnett’s test vs. MYO19898–970-GFP coexpressed with mchr-Miro2 wild type). (d) FRAP analysis of MYO19898–970-GFP with wild type or GTP-state mutants shows similar exchange kinetics of MYO19898–970-GFP for all three conditions. For the mchr-Miro2 T18N, this is likely due to endogenous Miro recruiting MYO19898–970-GFP. Numbers at the base of the bars indicate the number of replicates. Error bars represent SEM. FRAP kinetic parameters are listed in Table 1

Techniques: Expressing, Mutagenesis, Staining

prk5 myc miro2 Search Results

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